Wink & Blink are first shown when the player clicks on them while their faces aren't shown yet. Their faces appear after they are clicked on the first time. The doors reveals they can't let Pajama Sam through into the next hallway unless he wins a game called The Brain Tickler.
After Sam completes the brain tickler mini-game, he becomes the Grand Champion and is allowed to go through Wink & Blink anytime he wishes.
Throughout the game after Sam's victory, Wink & Blink will have minor arguments about being host and asking questions on the Brain Tickler mini-game though they are forced to postpone their arguments whenever they hear Sam coming upstairs or downstairs to them.
When Sam is invisible he will become visible again once he reaches the top of the stairs while Wink & Blink both shock in surprise of his appearance.